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Immunostaining of Dissected Zebrafish Embryonic Heart

Immunostaining of Dissected Zebrafish Embryonic Heart

Published on: January 10, 2012

Epigenomics and transcriptomics profiles of developing zebrafish heart cells.

Gulrez Chahal^1,2,3, Michael P Eichenlaub¹, Markus Tondl¹

¹Australian Regenerative Medicine Institute and Systems Biology Institute Australia, Monash University, Clayton, Victoria, 3800, Australia.

Scientific Data

|October 7, 2025

Summary

Researchers identified cardiac-specific cis-regulatory elements (cREs) in zebrafish, crucial for heart development. This resource aids in understanding gene regulation and congenital heart defects.

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Isolation and Characterization of Single Cells from Zebrafish Embryos

Isolation and Characterization of Single Cells from Zebrafish Embryos

Published on: March 12, 2016

Area of Science:

Genetics
Developmental Biology
Cardiovascular Research

Background:

cis-Regulatory elements (cREs) control gene expression, but their activity varies by cell type.
Understanding cell-type-specific cREs is vital for studying development and disease, particularly in the heart.
Zebrafish offer a valuable model for studying heart development due to conserved features with humans.

Purpose of the Study:

To create a comprehensive cardiomyocyte-specific repertoire of cREs from zebrafish larvae.
To identify genes regulated by these cREs during heart development.
To provide a resource for studying epigenetic and transcriptomic mechanisms in heart development and congenital heart defects.

Main Methods:

Combined in vivo transcriptomics and epigenetic profiling in zebrafish larvae.
Bioinformatic analysis to identify cardiomyocyte-specific cREs and associated genes.
Transgenic reporter assays to validate identified cREs, specifically for popdc2 and bmp10 genes.

Main Results:

A comprehensive repertoire of cardiomyocyte-specific cREs from zebrafish larvae was generated.
Identified cREs associated with key genes involved in heart development.
Validated specific cREs linked to popdc2 and bmp10 genes using transgenic reporter assays.

Conclusions:

The study presents a valuable, reproducible resource of cardiomyocyte-specific cREs.
This resource provides insights into the epigenetic and transcriptomic regulation of heart development.
The findings contribute to understanding the mechanisms underlying congenital heart defects.