Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

6.2K
Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
6.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Restoring TRPV4-KCa2.3 Coupling to Treat Pulmonary Arterial Hypertension.

Circulation research·2026
Same author

TRPV4 Promotes Vascular Calcification by Directly Associating With and Activating β-Catenin.

Arteriosclerosis, thrombosis, and vascular biology·2025
Same author

The gut microbiota-derived metabolite indole-3-propionic acid enhances leptin sensitivity by targeting STAT3 against diet-induced obesity.

Clinical and translational medicine·2024
Same author

Single-Cell RNA-Seq Reveals Coronary Heterogeneity and Identifies CD133<sup>+</sup>TRPV4<sup>high</sup> Endothelial Subpopulation in Regulating Flow-Induced Vascular Tone in Mice.

Arteriosclerosis, thrombosis, and vascular biology·2024
Same author

Correction: Silybin induces endothelium-dependent vasodilation via TRPV4 channels in mouse mesenteric arteries.

Hypertension research : official journal of the Japanese Society of Hypertension·2022
Same author

Silybin induces endothelium-dependent vasodilation via TRPV4 channels in mouse mesenteric arteries.

Hypertension research : official journal of the Japanese Society of Hypertension·2022

Related Experiment Video

Updated: Jan 15, 2026

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

10.0K

Indole-3-Propionic Acid Improves Vascular Function in High-Fat Diet-Induced Obese Mice via eNOS.

Shaying Yang1, Zhiwei Wang1, Xin Wen1

  • 1Department of Pharmacology, Wuxi School of Medicine, Jiangnan University, Wuxi, China.

Clinical and Experimental Pharmacology & Physiology
|October 8, 2025
PubMed
Summary

Indole-3-propionic acid (IPA) improves vascular function and lowers blood pressure in obese mice by reducing inflammation and increasing nitric oxide (NO) production. This metabolite offers potential for treating obesity-related cardiovascular issues.

Keywords:
NOeNOShypertensionindole‐3‐propionic acidinflammatory cytokinesobesityvasodilation

More Related Videos

Assessment of Vascular Tone Responsiveness using Isolated Mesenteric Arteries with a Focus on Modulation by Perivascular Adipose Tissues
08:41

Assessment of Vascular Tone Responsiveness using Isolated Mesenteric Arteries with a Focus on Modulation by Perivascular Adipose Tissues

Published on: June 3, 2019

10.3K
Author Spotlight: Exploring the Relationship Between Lipotoxicity and HFpEF
03:42

Author Spotlight: Exploring the Relationship Between Lipotoxicity and HFpEF

Published on: March 29, 2024

2.0K

Related Experiment Videos

Last Updated: Jan 15, 2026

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

10.0K
Assessment of Vascular Tone Responsiveness using Isolated Mesenteric Arteries with a Focus on Modulation by Perivascular Adipose Tissues
08:41

Assessment of Vascular Tone Responsiveness using Isolated Mesenteric Arteries with a Focus on Modulation by Perivascular Adipose Tissues

Published on: June 3, 2019

10.3K
Author Spotlight: Exploring the Relationship Between Lipotoxicity and HFpEF
03:42

Author Spotlight: Exploring the Relationship Between Lipotoxicity and HFpEF

Published on: March 29, 2024

2.0K

Area of Science:

  • Metabolomics
  • Cardiovascular Physiology
  • Gut Microbiome Research

Background:

  • Obesity is linked to vascular dysfunction and hypertension.
  • The role of gut microbiota metabolite indole-3-propionic acid (IPA) in obesity-related vascular issues is unclear.
  • This study investigates IPA's effects on mesenteric vascular function and blood pressure in obese mice.

Purpose of the Study:

  • To determine the impact of IPA on mesenteric vascular endothelial function.
  • To assess IPA's effect on blood pressure regulation in diet-induced obesity (DIO).
  • To elucidate the mechanisms underlying IPA's vascular effects.

Main Methods:

  • Diet-induced obesity (DIO) model established in C57BL/6 mice.
  • DIO mice treated daily with IPA (20 mg/kg) for 6 weeks.
  • Assessed inflammatory markers, nitric oxide (NO) levels, and mesenteric artery vasodilation; monitored blood pressure.

Main Results:

  • IPA treatment reduced inflammatory cytokines in endothelial cells.
  • IPA increased NO levels and enhanced endothelium-dependent vasodilation in mesenteric arteries.
  • IPA promoted NO production via eNOS phosphorylation through the PI3K/Akt pathway, regulating blood pressure.

Conclusions:

  • IPA exhibits vascular protective effects in obesity.
  • IPA enhances endothelial function and lowers blood pressure in obese mice.
  • IPA presents a potential therapeutic strategy for obesity-related cardiovascular diseases.