LYMPHOCYTE SUBSET DISTRIBUTION AFTER COMBINED CHEMO- AND RADIOTHERAPY IN PATIENTS WITH CANCER OF THE ORAL CAVITY,
M Vorobyov1, L Zvarych2, D Bazyka2
1Municipal Non-Profit Enterprise «Zaporizhzhia Regional Antitumor Center» Zaporizhzhia Regional Council, Zaporizhzhia, Ukraine.
Aim:
To study the prognostic value of the lymphocyte subset distribution to predict the overall survival and its association with the clinicopathologic characteristics and treatment in patients with cancer of the oral cavity, oropharynx, and laryngopharynx.
Materials And Methods:
44 patients were examined. Immunophenotyping of lymphocyte subsets was performed in peripheral blood samples using flow cytometry. The lymphocyte subset distribution was analyzed depending on the clinicopathological characteristics and treatment outcome, as well as the overall survival.
Results:
The changes in CD3+ T-cells and CD3+57+ NKT counts were associated with the sex of the patients, TCRαβ+ T-cells - with the stage of the disease, CD4+8+T-cells and CD3-16+57+ NK - with the tumor size and differentiation grade, and CD3+HLA-DR+,CD8+ T-cells, and CD4+/CD8+ ratio - with lymph node involvement. The content of CD3+HLA-DR+ and TCRαβ+ T-cells, CD3-16+57+ NK, and CD3+57+ NKT differed in patients depending on the tumor location. There were changes in CD19+ and HLA-DR+ B-cells, CD3+, CD4+, CD4+25+ and TCRαβ+ T-cells, CD3-CD16+57+ NK, and CD3+57+ NKT during treatment, with the most pronounced changes after the first stage of RT. The relative number of CD3+HLA-DR+ and tumor size T4 influenced the overall survival of patients ((HR = 0.798, 95% CI, 0.658-0.967, p = 0.021) and (HR = 3.015, 95% CI, 1.303-6.975, p = 0.009), respectively).
Conclusion:
Parameters of lymphocyte subsets can be promising prognostic markers.
More Related Videos
Related Concept Videos
01:16Primary Lymphoid Organs
The red bone marrow is a soft, spongy tissue nestled in the interior of long bones such as the humerus and femur. It is the site...
02:56Treatment Resistant Cancers
01:15Secondary Lymphoid Organs
The spleen is a vital organ in the lymphatic system, nestled in the upper left side of the abdomen. It is composed of two primary regions: the red pulp and the white pulp, each having distinct functions. The red pulp performs a significant role in blood filtration. It efficiently purges the blood of old or damaged red blood cells and...
02:49Cancer Therapies
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
01:18Lymphoid Cells and Tissues
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
02:50Combination Therapies and Personalized Medicine
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...