Related Experiment Video

Updated: Jan 15, 2026

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice
07:25

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

Published on: September 25, 2019

7.3K

Tissue-resident memory CD8+T cells might enhance HBV DNA clearance in CHB patients with MASLD complication and normal

Chao Cai1, Wen-Xuan Shang1, Si-Jie Zheng1

  • 1Department of Infectious Diseases, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Frontiers in Cellular and Infection Microbiology
|October 8, 2025
PubMed

Insights

Metabolic dysfunction-associated steatotic liver disease (MASLD) aids hepatitis B virus (HBV) DNA clearance in chronic hepatitis B (CHB) patients with normal ALT. MASLD enhances immune cell activity, promoting viral clearance.

Area of Science:

  • Hepatology
  • Immunology
  • Virology

Background:

  • Hepatitis B virus (HBV) infection remains a global health concern, especially in patients with normal alanine aminotransferase (ALT) levels.
  • Metabolic dysfunction-associated steatotic liver disease (MASLD) is increasingly prevalent in chronic hepatitis B (CHB) patients, complicating management.
  • This study examines the impact of MASLD on HBV DNA clearance in CHB patients with normal ALT.

Purpose of the Study:

  • To investigate the relationship between MASLD and HBV DNA clearance in CHB patients with normal ALT.
  • To explore the immunological mechanisms underlying HBV DNA clearance in the presence of MASLD.

Main Methods:

  • Retrospective analysis of 403 CHB patients with normal ALT who received antiviral therapy.
  • Diagnosis of MASLD based on liver biopsy findings.
  • Single-cell data analysis of relevant datasets (GSE192740, GSE182159).

Main Results:

  • CHB patients with MASLD demonstrated a higher likelihood of achieving HBV DNA clearance.
  • Increased proportion of CD8+Teff-GZMH cells and enhanced interactions among CD8+ Trm-CD69 cells were observed in the MASLD group.
  • Elevated Monocyte-THBS1 proportion and activated CCL-CCR pathways were noted in patients with MASLD.

Conclusions:

  • Monocyte-THBS1 cells may activate CD8+ Trm-CD69 T cells, enhancing immune response via the CCL-CCR signaling axis.
  • This immune activation facilitates immune cell recruitment and improves hepatitis B virus (HBV) clearance.
  • MASLD may play a beneficial role in managing CHB by promoting viral clearance through immune modulation.
Abstract

Keywords:
antiviral therapychronic hepatits Bliver biopsymetabolic dysfunction-associated steatotic liver diseasesingle-cell RNA sequencing

More Related Videos

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix10:37

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Published on: October 20, 2021

3.4K
Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

10.2K

Related Concept Videos

Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

Overview
83.5K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
14.7K
Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
1.7K
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
6.5K