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Updated: Jan 6, 2026
A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System
Published on: August 30, 2019
Characterization of the gut microbiome in Wuhuang pigs and their crossbred offspring
Zhijuan Yan1,2,3, Yiting Yang1,2,3, Xinghong Yu1,2,3
1Farm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Introduction:
As an indigenous Chinese breed, Wuhuang pigs are valued for their stress resistance, tolerance to coarse feed, and high lean meat yield, while Berkshire pigs serve as ideal sires due to superior meat quality and early maturity. To explore the microbial basis of hybrid vigor in these breeds, we compared the gut microbiota of purebred Wuhuang pigs and Wuhuang-Berkshire hybrids.
Methods:
Microbial composition was assessed via 16S rDNA sequencing, and predictive functional profiling was performed using PICRUSt2 analysis.
Results:
Hybrids exhibited significantly increased microbial α-diversity and altered β-diversity. Notably, hybrid ceca were enriched with probiotic genera involved in fiber degradation and short-chain fatty acid (SCFA) production-such as Prevotella, Ruminococcus, Lachnospiraceae, and Roseburia-accompanied by a higher Firmicutes-to-Bacteroidetes ratio and strengthened microbial network connectivity. Predictive functional profiling further revealed significantly elevated activity in hybrid pigs for key metabolic pathways including tryptophan synthesis, pyridoxal salvage, and galacturonic acid metabolism (FDR < 0.05).
Discussion:
These results imply that hybrid animals leverage enriched probiotic consortia to augment nutrient metabolism and immune function, thereby supporting improved stress resilience and feed efficiency. This study provides potential microbial targets for the future genetic improvement of indigenous pig breeds.
