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Biomineralized gold nanoparticle-based colorimetric sensing platform for ascorbic acid detection.

Zhuqing Wang1, Junyuan Zhang1, Yuetong Ma2

  • 1Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|October 8, 2025
PubMed
Summary

A new colorimetric sensor using gold nanoparticles (AuNPs) enables rapid, visual detection of ascorbic acid (AA). This method offers a simple, cost-effective way to monitor antioxidants in biological samples.

Keywords:
Ascorbic acidBiomimetic mineralizationColorimetric assayGold nanoparticlesOxidase-like activity

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Area of Science:

  • Nanomaterials Science
  • Analytical Chemistry
  • Biochemistry

Background:

  • Ascorbic acid (AA) is crucial for redox homeostasis and disease prevention.
  • Rapid and precise detection methods for AA are essential.
  • Existing methods may lack speed, simplicity, or cost-effectiveness for on-site monitoring.

Purpose of the Study:

  • To develop a novel colorimetric sensing platform for the visual and rapid detection of ascorbic acid (AA).
  • To utilize bioinspired mineralization-synthesized gold nanoparticles (AuNPs) for enhanced sensing capabilities.
  • To establish a sensitive, selective, and cost-effective method for antioxidant monitoring.

Main Methods:

  • Synthesis of gold nanoparticles (AuNPs) via bioinspired mineralization.
  • Utilizing AuNPs' oxidase-like activity to catalyze TMB oxidation.
  • Monitoring the inhibition of TMB oxidation by ascorbic acid (AA) through colorimetric changes and absorbance measurements at 650 nm.

Main Results:

  • The developed AuNPs showed uniform morphology and significant oxidase-like activity.
  • Ascorbic acid (AA) inhibited TMB oxidation, leading to a visually detectable color change within 5 minutes.
  • A linear relationship was observed between absorbance decrease and AA concentration (1-60 μM, R²=0.999) with a detection limit of 0.27 μM.
  • The method demonstrated excellent selectivity and successful application in fruit sample analysis with high recovery rates (98.5-106.1%).

Conclusions:

  • The proposed colorimetric sensing strategy offers a simple, cost-effective, and rapid method for on-site AA detection.
  • The bioinspired AuNPs provide a sensitive platform for monitoring antioxidants in biological samples.
  • This approach presents new insights for developing advanced antioxidant detection systems.