Modular Virus Capsid Coatings for Biocatalytic DNA Origami Nanoreactors

Area of Science:

• Biotechnology
• Nanotechnology
• Biochemistry

Background:

• Protein cages and DNA origami are self-assembling nanocompartments for enzyme sequestration.
• They mimic natural biocatalytic compartmentalization but face challenges in controlled enzyme loading and substrate flux.
• Combining these nanomaterials offers a potential solution to overcome these limitations.

Purpose of the Study:

• To develop a modular platform by assembling virus capsid proteins onto enzyme-loaded DNA origami nanoreactors.
• To investigate the impact of capsid protein properties on substrate uptake and enzyme protection.
• To explore the potential for targeted delivery via surface functionalization.

Main Methods:

• Assembly of virus capsid proteins onto enzyme-loaded DNA origami nanoreactors.
• Characterization of substrate uptake selectivity based on capsid protein type and amount.
• Assessment of enzyme protection against degradation.
• Functionalization of nanoreactor surfaces with antibody fragments.

Main Results:

• Demonstrated size-selective substrate uptake, modulated by capsid protein composition and quantity.
• Confirmed protection of the encapsulated biocatalytic unit from degradation.
• Successfully functionalized nanoreactor surfaces for potential targeting.
• Established a modular platform for investigating capsid protein properties.

Conclusions:

• The combined DNA origami and virus capsid protein approach creates a versatile nanoreactor platform.
• This platform enables controlled enzyme loading, selective substrate permeation, and enhanced stability.
• The modularity facilitates biomedical applications and research into capsid protein physicochemical properties.