Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Phylum Actinobacteria01:30

Bacterial Phylum Actinobacteria

613
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
613
Bacterial Phylum Firmicutes01:27

Bacterial Phylum Firmicutes

923
Firmicutes is a diverse phylum of Gram-positive bacteria characterized by a low GC content in their genomes. This phylum includes organisms with monoderm or diderm cell envelopes, highlighting a complex evolutionary history. Firmicutes comprises several major orders, including Lactobacillales, Clostridiales, and Bacillales, which exhibit remarkable diversity in their morphology, metabolism, and ecological roles.The order Lactobacillales includes lactic acid bacteria, which are fermentative...
923
Bacterial Phylum Bacteroidota01:26

Bacterial Phylum Bacteroidota

581
The phylum Bacteroidota includes over 700 species classified into four primary orders: Bacteroidales, Cytophagales, Flavobacteriales, and Sphingobacteriales. These gram-negative, non-sporulating rods exhibit saccharolytic capabilities and can be aerobic or fermentative, encompassing obligate aerobes, facultative aerobes, and obligate anaerobes. Many species display gliding motility, though some are nonmotile or use flagella. The genus Bacteroides is well-studied due to its significant role in...
581
Cytoskeletal Proteins in Bacteria01:29

Cytoskeletal Proteins in Bacteria

4.1K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
4.1K
Bacterial Phylum Tenericutes01:24

Bacterial Phylum Tenericutes

446
The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
446
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

518
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
518

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lacticaseibacillus rhamnosus Lcr35 Stimulates Epithelial Vaginal Defenses upon Gardnerella vaginalis Infection.

Infection and immunity·2022
Same journal

A lysate of a multi-strain probiotic formulation inhibits cell growth and oxidative phosphorylation in colorectal cancer cells via a ceramide-mediated mechanism.

Beneficial microbes·2026
Same journal

Gut microbial differences and function in infants with gastroschisis: a pilot prospective cohort study.

Beneficial microbes·2026
Same journal

Infant gut microbiota composition and metabolism can be influenced by extensively hydrolysed casein in vitro.

Beneficial microbes·2026
Same journal

Gut commensal Bacteroides faecichinchillae, a potential novel candidate of next-generation probiotics targeting type 2 diabetes.

Beneficial microbes·2026
Same journal

Ultra long-lived plasma cells in the human small intestine produce microbiota-reactive IgA antibodies.

Beneficial microbes·2026
Same journal

Engraftment of staphylococcal strains on human skin can competitively displace native staphylococci.

Beneficial microbes·2026
See all related articles

Related Experiment Video

Updated: Jan 15, 2026

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry
05:19

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry

Published on: September 29, 2023

1.2K

Aggregation in lactobacilli: an unexplored dimension.

M Kaur1, C Forestier1, S Miquel1

  • 1LMGE, Université Clermont Auvergne, CNRS, F-63000 Clermont-Ferrand, France.

Beneficial Microbes
|October 8, 2025
PubMed
Summary
This summary is machine-generated.

Autoaggregation in Lactobacillus is key for host colonization and probiotic function. This review details its molecular basis, surface factors, and environmental influences, distinguishing it from biofilm formation.

More Related Videos

Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
08:04

Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining

Published on: February 8, 2019

9.1K
The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
04:40

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

Published on: June 16, 2022

9.3K

Related Experiment Videos

Last Updated: Jan 15, 2026

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry
05:19

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry

Published on: September 29, 2023

1.2K
Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
08:04

Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining

Published on: February 8, 2019

9.1K
The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
04:40

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

Published on: June 16, 2022

9.3K

Area of Science:

  • Microbiology
  • Bacterial Physiology

Background:

  • Autoaggregation is a critical Lactobacillus trait influencing host colonization, pathogen exclusion, and probiotic efficacy.
  • It is often linked to initial biofilm formation but can also occur independently.

Purpose of the Study:

  • To review the molecular mechanisms, surface factors, and environmental cues governing Lactobacillus autoaggregation.
  • To differentiate autoaggregation from biofilm formation and explore their interplay.
  • To highlight the role of autoaggregation in host-microbe interactions and its potential as a selection marker for probiotics.

Main Methods:

  • Literature review of studies on Lactobacillus autoaggregation.
  • Analysis of molecular mechanisms, including surface proteins (S-layer, aggregation-promoting factors) and exopolysaccharides.
  • Examination of environmental influences on aggregation behavior.

Main Results:

  • Autoaggregation is modulated by surface proteins, exopolysaccharides, pili, and environmental factors.
  • Aggregation enhances mucosal adhesion, immune modulation, and competitive exclusion of pathogens.
  • Autoaggregation can occur independently of, and sometimes inversely to, biofilm production.

Conclusions:

  • Autoaggregation is a crucial, multifaceted trait in Lactobacillus with significant implications for health and disease.
  • It serves as a promising selection marker for next-generation probiotics and live biotherapeutics.
  • Standardized methods are needed to fully understand the complex relationship between bacterial surface architecture and lifestyle strategies like aggregation and biofilm formation.