Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

6.7K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
6.7K
Molecular Spectroscopy: Absorption and Emission01:14

Molecular Spectroscopy: Absorption and Emission

4.3K
Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels.  Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.
4.3K
UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

6.6K
The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
6.6K
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

3.4K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
3.4K
Atomic Spectroscopy: Absorption, Emission, and Fluorescence01:23

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

2.6K
Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...
2.6K
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

2.7K
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Monolithic Integration of Sensing, Computing, and Storage in an Anomalous Hall Effect-Based Neuromorphic Device.

ACS applied materials & interfaces·2025
Same author

Effects of Dietary Application of the Probiotic <i>Lactobacillus paracasei</i> N1115 on Growth, Hepatic Antioxidant and Immune Biomarkers, and Intestinal Microbiota and Histology of Hybrid Sturgeon (<i>Acipenser baerii</i> ♀ × <i>A. schrenckii</i> ♂).

Aquaculture nutrition·2025
Same author

Unveiling the black box: Multi-omics reveal how biochar supercharges synthetic biofilms for superior bioremediation.

Journal of hazardous materials·2025
Same author

Knowledge, attitudes, and practices of parents of pediatric patients undergoing eye surgery under general anesthesia: a cross-sectional study.

Scientific reports·2025
Same author

P4HA3 drives cervical cancer lymphatic metastasis by facilitating ACLY-mediated ferroptosis resistance.

Cell death and differentiation·2025
Same author

Efficacy of Baduanjin Exercise for Sarcopenia in Older Adults: A 24-Week Randomized Controlled Trial.

Journal of cachexia, sarcopenia and muscle·2025

Related Experiment Video

Updated: Jan 15, 2026

In situ Grazing Incidence Small Angle X-ray Scattering on Roll-To-Roll Coating of Organic Solar Cells with Laboratory X-ray Instrumentation
06:49

In situ Grazing Incidence Small Angle X-ray Scattering on Roll-To-Roll Coating of Organic Solar Cells with Laboratory X-ray Instrumentation

Published on: March 2, 2021

6.7K

A refinable three-parameter equation for phenomenological absorption in quantitative electron microscopy -

Philip N H Nakashima1, Tianyu Liu2, Andrew E Smith3

  • 1Department of Materials Science and Engineering Monash University Victoria3800 Australia.

Journal of Applied Crystallography
|October 9, 2025
PubMed
Summary

A new three-term equation models electron beam absorption in materials for transmission electron microscopy simulations. This equation accurately reproduces scattering factors and is adaptable for advanced quantitative convergent-beam electron diffraction analysis.

Keywords:
differential QCBEDelectron scattering calculationsinelastic scattering factorsphenomenological absorptionquantitative convergent-beam electron diffractionquantitative transmission electron microscopy

More Related Videos

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
07:38

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

2.9K
Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research
08:12

Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research

Published on: February 16, 2024

15.1K

Related Experiment Videos

Last Updated: Jan 15, 2026

In situ Grazing Incidence Small Angle X-ray Scattering on Roll-To-Roll Coating of Organic Solar Cells with Laboratory X-ray Instrumentation
06:49

In situ Grazing Incidence Small Angle X-ray Scattering on Roll-To-Roll Coating of Organic Solar Cells with Laboratory X-ray Instrumentation

Published on: March 2, 2021

6.7K
Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
07:38

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

2.9K
Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research
08:12

Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research

Published on: February 16, 2024

15.1K

Area of Science:

  • Materials Science
  • Physics
  • Crystallography

Background:

  • Quantitative convergent-beam electron diffraction (QCBED) simulations require accurate models of electron scattering.
  • Existing models, like the ATOM subroutine, are effective but may need refinement for unfiltered diffraction patterns.

Purpose of the Study:

  • To derive a new three-term phenomenological equation for electron beam absorption in materials.
  • To improve the accuracy of transmission electron microscopy (TEM) simulations, particularly for differential QCBED.

Main Methods:

  • Developed a three-term equation for phenomenological absorption.
  • Validated the equation against the ATOM subroutine's inelastic scattering factors across a wide range of elements and parameters.
  • Investigated the refinement of equation coefficients for local and non-local scattering contributions.

Main Results:

  • The derived equation accurately reproduces inelastic scattering factors (within a few percent) generated by the ATOM subroutine.
  • The equation is applicable across elements Z=1-98, Debye-Waller parameters (0.05-2.0 Ų), scattering angles (0-6.0 Å⁻¹), and electron energies (1 keV-1 MeV).
  • The equation's coefficients can be refined for improved suitability in unfiltered differential QCBED pattern matching.

Conclusions:

  • The new three-term equation provides a robust and adaptable model for electron beam absorption in materials.
  • This model enhances the capabilities of quantitative TEM simulations, especially for unfiltered QCBED analysis.
  • The refined coefficients offer greater flexibility in modeling scattering phenomena for advanced materials characterization.