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Placenta-Derived Proteins in Preeclampsia: A Human In Vivo Study
Ane Cecilie Westerberg1, Maren-Helene Langeland Degnes1,2, Ina Jungersen Andresen1
1Division of Obstetrics and Gynecology, Department of Obstetrics (A.C.W., M.-H.L.D., I.J.A., H.B.S., T.H., M.C.P.R., T.M.M.), Oslo University Hospital Rikshospitalet, Norway.
Researchers identified 620 placenta-derived proteins in preeclampsia, with 345 unique to the condition. Several proteins showed potential as early biomarkers for preeclampsia, aiding in diagnosis and management.
Area of Science:
- Obstetrics and Gynecology
- Proteomics
- Biomarker Discovery
Background:
- Preeclampsia is characterized by placental dysfunction, leading to altered protein release into maternal circulation.
- Identifying these placenta-derived proteins is challenging due to the inaccessibility of the placenta during pregnancy.
Purpose of the Study:
- To identify placenta-derived proteins in preeclampsia.
- To characterize their associated biological processes.
- To assess their potential as biomarkers for early detection.
Main Methods:
- Utilized a 4-vessel sampling method to collect blood from placental vessels during cesarean section.
- Quantified proteins using the SomaLogic 5000-plex platform.
- Analyzed samples from a separate prospective pregnancy cohort.
Main Results:
- Identified 620 placenta-derived proteins: 229 in both healthy and preeclamptic pregnancies, 345 in preeclampsia only, and 46 in healthy controls only.
- Enriched biological processes in preeclampsia-associated proteins included oxidative stress and cellular turnover.
- In prospective samples, 21 proteins showed altered levels >3 weeks before delivery, with 11 demonstrating promising predictive performance (AUC >0.7).
Conclusions:
- A unique in vivo sampling method successfully identified placenta-derived proteins in preeclampsia.
- Several identified proteins exhibit significant potential as early biomarkers for preeclampsia, improving diagnostic capabilities.
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05:31Author Spotlight: Modeling an Aspect of Preeclampsia in Female Mice Using Hypoxic Human Placenta-Derived Small Extracellular Vesicles
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