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Updated: Aug 3, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Published on: November 23, 2016
An activity-based probe library for identifying promiscuous amide hydrolases
Chathuri J Kombala1, Jared O Kroll1, Lucas C Webber1
1Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, USA. krbrandvold@gmail.com.
Abstract:
A fluorogenic substrate library was developed to detect amide hydrolase activity in soil-derived chitin-degrading bacteria. Hit compounds were converted into pull-down probes for chemoproteomic enrichment, identifying previously unannotated proteins now linked to putative hydrolases. This approach prioritizes candidate hydrolases for further experimental validation with potential applications in the environment, biomanufacturing, and medicine.

