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Updated: Jan 15, 2026

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
Published on: March 15, 2019
Delineating inter- and intra-antibody repertoire evolution with AntibodyForests
Daphne van Ginneken1, Valentijn Tromp1, Lucas Stalder2
1Center for Translational Immunology, University Medical Center Utrecht, Lundlaan 6, 3584 EA Utrecht, The Netherlands.
AntibodyForests is new software for analyzing B cell evolution. It helps researchers understand antibody repertoire dynamics and somatic hypermutation using advanced computational methods.
Area of Science:
- Immunology
- Computational Biology
- Bioinformatics
Background:
- Advancements in immune repertoire sequencing and single-cell technologies offer new ways to study B cell evolution.
- Analyzing B cell repertoire dynamics requires specialized software for complex inter- and intra-repertoire analyses.
Purpose of the Study:
- To introduce AntibodyForests, a novel R package for comprehensive B cell repertoire analysis.
- To enable detailed study of B cell lineage inference and antibody evolution dynamics.
Main Methods:
- Utilizes protein language models and protein structure analysis.
- Infers B cell lineages and quantifies repertoire evolution.
- Analyzes somatic hypermutation within antibody repertoires.
Main Results:
- Provides software for inferring B cell lineages.
- Enables quantification of inter- and intra-antibody repertoire evolution.
- Facilitates somatic hypermutation analysis using advanced computational techniques.
Conclusions:
- AntibodyForests offers a powerful tool for dissecting B cell repertoire complexity.
- The software supports in-depth analysis of immune responses and B cell evolution.
- Facilitates the application of AI and advanced computational methods in immunology research.
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