A Supramolecular Material for Selective Fluorescence-Based Detection of the Neuromuscular Blocker Rocuronium Bromide.

Area of Science:

• Analytical Chemistry
• Biomedical Engineering
• Chemical Sensing

Background:

• Rocuronium Bromide (RcBr) is a critical neuromuscular blocker in anesthesia.
• Clinical monitoring of RcBr is important but analytically challenging.
• Existing analytical methods for RcBr are limited.

Purpose of the Study:

• To develop a novel fluorescence-based sensor for Rocuronium Bromide.
• To enable sensitive and selective detection of RcBr.
• To explore potential clinical applications for neuromuscular blockade monitoring.

Main Methods:

• Utilized Thioflavin-T (ThT) aggregates templated by γ-sulfated cyclodextrin (γ-SCD).
• Employed a competitive displacement assay based on RcBr's binding affinity for γ-SCD.
• Measured fluorescence turn-off response upon RcBr detection.

Main Results:

• Achieved submicromolar sensitivity with a limit of detection (LOD) of approximately 0.2 ± 0.02 μM.
• Demonstrated rapid response time (<1 minute).
• Exhibited high selectivity against common biological interferents and functionality in diluted human serum.

Conclusions:

• Developed a novel optical sensor for Rocuronium Bromide.
• The sensor offers high sensitivity, rapid response, and selectivity.
• This approach shows promise for advancing neuromuscular blockade monitoring in clinical settings.