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Digestive and fermentation characteristics of esterified starches based on digestion kinetics, microbiome and
Tong Wang1, Chang Liu1, Rongrong Ma1
1State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Abstract:
Esterified starch (ES) is one of the most common forms of resistant starch employed in the food industry. In this study, the digestive and fermentation properties of ES with different substitution groups were investigated by digestion kinetics, microbiome and metabolomics. The introduction of esterification groups slows down the digestion rate of starch and alters the crystal structure and molecular weight distribution. Following in vitro fermentation, ES significantly reduced the relative abundance of opportunistic pathogens. Furthermore, predicted analysis indicated that ES enhances the microbiota's capabilities in terms of pyruvate metabolism, and [EC: 1.2.4.1] and [EC: 2.3.1.12] were found to be over-expressed. The intervention of ES mainly regulates the levels of metabolites related to the metabolism and synthesis pathways of amino acids. Structural equation modeling indicates that, the molecular weight distribution of ES can significantly induce changes in the composition of the gut microbiota, and further affect metabolic enzymes and metabolites.
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