Human brain slice cultures: translational applications and ethical considerations

Human organotypic brain slice cultures have emerged as a pivotal tool to study the complexities of the human brain. Human organotypic brain slice cultures preserve the structural integrity, cellular diversity, and vascular networks of living brain tissue, maintaining in vivo characteristics. This advancement enables accurate temporal modeling of neurological diseases and facilitates precise experimental manipulations, accelerating therapeutic development. However, their use raises important ethical and philosophical considerations, including issues of donor consent and the potential for neural activity that prompts questions about consciousness. This study outlines these emerging concerns, emphasizing the need for guidelines that balance scientific innovation with ethical responsibility, particularly in relation to donor consent, transparency, and long-term use of living human tissue.