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Updated: Jan 15, 2026
09:33
Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
Published on: May 12, 2017
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PARP10 is critical for stress granule initiation
Aravinth Kumar Jayabalan1, Krishna Bhambhani1,2, Anthony Kl Leung3,4,5,6
1Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.
Life Science Alliance
|October 9, 2025
Summary
Researchers identified Poly (ADP-ribose) polymerase 10 (PARP10) as crucial for stress granule (SG) assembly. PARP10 regulates SG formation by modifying G3BP1, impacting cellular stress responses.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Stress granules (SGs) are cytoplasmic condensates involved in cellular stress responses.
- SG formation is linked to diseases like viral infections, cancer, and neurodegeneration.
- ADP-ribosylation is known to play a role in SG assembly, but the responsible enzyme was unknown.
Purpose of the Study:
- To identify the specific ADP-ribosyltransferase enzyme involved in stress granule assembly.
- To elucidate the mechanism by which this enzyme regulates SG formation.
- To understand the role of ADP-ribosylation in the kinetics and composition of stress granules.
Main Methods:
- Systematic knockdown of human ADP-ribosyltransferase family members.
- Live-cell imaging to observe stress granule assembly dynamics.
- Identification of stress granule components and their modifications, including G3BP1.
Main Results:
- Poly (ADP-ribose) polymerase 10 (PARP10) was identified as essential for stress granule assembly.
- PARP10 regulates the kinetics of initial stress granule formation.
- G3BP1 was identified as a direct substrate of PARP10, and PARP10 influences SG composition and eIF2α phosphorylation.
Conclusions:
- PARP10 is a pivotal enzyme in initiating stress granule assembly.
- ADP-ribosylation by PARP10 acts as a rate-limiting step in stress granule formation.
- PARP10-mediated regulation of G3BP1 is critical for stress granule biogenesis and cellular stress response.
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