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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Acrylamide-mediated errors in the cell cycle regulation are associated with altered TORC2 signaling in

Alica Navrátilová1, Marek Kovár2, Lucia Klongová3

  • 1Institute of Nutrition and Genomics , Slovak University of Agriculture , Nitra, Slovakia.

Scientific Reports
|October 9, 2025
PubMed

Insights

Acrylamide exposure causes cell damage by disrupting TORC2 signaling and cell cycle regulation. This study reveals acrylamide

Area of Science:

  • Cell Biology
  • Toxicology
  • Molecular Biology

Background:

  • Acrylamide (AA) is a toxic compound linked to severe health risks.
  • AA exposure induces oxidative stress, affecting cellular functions and gene expression.
  • Cell cycle regulation and division are critical processes impacted by AA.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying acrylamide-induced cellular damage.
  • To explore the role of TORC2 signaling in response to acrylamide exposure.
  • To elucidate the link between AA, cellular homeostasis disruption, and carcinogenesis.

Main Methods:

  • Gene expression analysis of key regulators involved in cell cycle and stress response pathways.
  • Investigated the impact of AA on TORC1 and TORC2 complexes.
  • Utilized S. pombe as a model organism to study AA effects.

Main Results:

  • AA exposure alters TORC2 signaling pathway components, including tor1, wat1, ste20, sin1, bit61, and gad8.
  • AA disrupts cell cycle regulation, affecting genes like cdc2, cdc13, and cdc25.
  • AA exposure leads to cell division defects and chromosome segregation errors.
  • Unexpectedly, AA did not affect the expression of sty1, a key MAPK pathway kinase in S. pombe.

Conclusions:

  • Acrylamide disrupts cellular homeostasis by altering TORC2 signaling and cell cycle regulation.
  • These disruptions ultimately contribute to the development of cancer.
  • The study provides novel insights into the carcinogenic mechanisms of acrylamide.
Keywords:
Schizosaccharomyces pombeAcrylamideCarcinogenesisCell cycleROSTORC2

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