Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

8.7K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.7K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

2.0K
2.0K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

6.5K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
6.5K
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

3.8K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
3.8K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.9K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.9K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.1K
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Network Formation Dynamics in Thiol-ene Crosslinked Hyaluronic Acid Hydrogels: Design Principles for In Vitro Tissue Models.

bioRxiv : the preprint server for biology·2026
Same author

Enzymatic Methods for Assembling and Modifying Hydrogel Biomaterials.

Regenerative engineering and translational medicine·2026
Same author

A robust and user-agnostic step-emulsion platform for scalable microgel fabrication.

bioRxiv : the preprint server for biology·2026
Same author

De novo design of protein nanoparticles with integrated functional motifs.

bioRxiv : the preprint server for biology·2026
Same author

Suspended Tissue Engineering with Assemblable Microfluidics (STEAM).

bioRxiv : the preprint server for biology·2025
Same author

Boolean Logic-Based Controlled Release of Bioactive Proteins with Diversified Inputs.

Angewandte Chemie (International ed. in English)·2025

Related Experiment Video

Updated: Jan 15, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.9K

Boolean logic-gated protein presentation through autonomously compiled molecular topology.

Ryan Gharios1, Murial L Ross2, Annabella Li1

  • 1Department of Chemical Engineering, University of Washington, Seattle, WA, USA.

Nature Chemical Biology
|October 9, 2025
PubMed
Summary

Researchers developed a new method for creating stimulus-responsive biomaterials using recombinant protein expression. This approach enables precise control over material behavior for applications in biosensing and drug delivery.

More Related Videos

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.9K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.8K

Related Experiment Videos

Last Updated: Jan 15, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.9K
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

12.9K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.8K

Area of Science:

  • Biomaterials Science
  • Chemical Biology
  • Synthetic Biology

Background:

  • Stimulus-responsive materials are crucial for advanced applications like biosensing and drug delivery.
  • Existing methods for creating complex responsive materials often involve difficult and inefficient multi-step organic syntheses.
  • Controlled molecular topology is key to designing materials that respond to specific input combinations.

Purpose of the Study:

  • To develop a scalable and efficient method for synthesizing topologically specified protein cargos for biomaterials.
  • To enable user-programmable Boolean logic for conditional release of cargo from biomaterials.
  • To demonstrate the versatility of this approach for various applications including multiplexed delivery and cellular localization.

Main Methods:

  • Utilized recombinant expression and chemical biology tools to create topologically specified protein cargos.
  • Integrated spontaneous intramolecular ligations for autonomous construct topology compilation during expression.
  • Employed orthogonal protease actuators to achieve user-programmable Boolean logic (AND, OR, YES gates).

Main Results:

  • Successfully synthesized advanced Boolean logic operators through a direct and scalable expression process.
  • Demonstrated all 17 possible logic outputs for protein release based on input combinations of three orthogonal protease actuators.
  • Achieved multiplexed delivery of three distinct biomacromolecules from hydrogels and conditional cargo liberation based on five inputs.

Conclusions:

  • This novel framework enables the scalable and direct synthesis of advanced, topologically defined protein cargos.
  • The developed system offers precise, logic-gate-controlled release of biomolecules from materials.
  • The approach has significant potential for applications in advanced drug delivery, biosensing, and cellular engineering.