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Updated: Jan 15, 2026

NanoDrop Microvolume Quantitation of Nucleic Acids

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

Quantifying Nanoparticles by DNA-Driven Nanochemical Titration.

Tong Xia¹, Ziyue Guo¹, Lei Song¹

¹Hefei National Research Center for Physical Sciences at the Microscale, Center for Bioanalytical Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui 230026, China.

|October 9, 2025

Summary

This summary is machine-generated.

Researchers developed a novel titration method to measure nanoparticle molar concentrations. This technique uses DNA-programmable assembly for accurate quantification, simplifying nanoparticle characterization.

Keywords:

DNA-programmable assembly chemical titration molar extinction coefficient nanoparticle dimer nanoparticle quantitation

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Concentration Determination of Nucleic Acids and Proteins Using the Micro-volume Bio-spec Nano Spectrophotometer

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Published on: February 17, 2011

Area of Science:

Nanotechnology
Analytical Chemistry
Biochemistry

Background:

Nanosynthesis provides diverse nanomaterials with advanced functionalities.
Accurate molar concentration measurement of complex nanoparticles remains a challenge.

Purpose of the Study:

To develop a general chemical method for determining nanoparticle molar extinction coefficients.
To enable accurate quantification of nanoparticles without prior compositional or morphological data.

Main Methods:

Utilized DNA-programmable assembly to drive nanoparticle interactions.
Formed 1:1 stoichiometric heterodimers between titrant and analyte nanoparticles.
Employed spectral deconvolution to calculate molar extinction coefficients.

Main Results:

Successfully determined molar extinction coefficients for various nanoparticles.
The method demonstrated robustness against nanoparticle loss (adsorption/precipitation).
Established a unified standard for nanoparticle molar extinction coefficients using gold nanospheres as common titrants.

Conclusions:

The developed titration-based method offers a reliable way to quantify nanoparticle molar concentrations.
This approach simplifies nanoparticle characterization and facilitates inter-laboratory comparisons.
The technique overcomes limitations of existing methods for complex nanomaterials.