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Related Experiment Video

Updated: Jan 15, 2026

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
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Real-Time Intracellular Bacterial Metabolism During Shigella Infection Using Hyperpolarized [2-13C]Pyruvate.

Thomas B Wareham Mathiassen1, Ke-Chuan Wang1, Sebastian Meier2

  • 1Center for Hyperpolarization in Magnetic Resonance, Department of Health Technology, Technical University of Denmark, Kgs. Lyngby, Denmark.

NMR in Biomedicine
|October 10, 2025
PubMed
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International journal of biological macromolecules·2025

Dynamic Nuclear Polarization-Nuclear Magnetic Resonance (dDNP-NMR) noninvasively tracks real-time bacterial metabolism within host cells. This method reveals Shigella acetate production in infected HeLa cells, advancing infectious disease research.

Area of Science:

  • Metabolic imaging
  • Infectious disease research
  • Cellular metabolism

Background:

  • Studying intracellular bacterial metabolism is difficult with traditional indirect methods.
  • Hyperpolarized magnetic resonance spectroscopy (MRS) offers noninvasive, real-time metabolic monitoring.
  • MRS can differentiate between host and pathogen metabolic activity.

Purpose of the Study:

  • To demonstrate the utility of dDNP-NMR for studying intracellular bacterial infections.
  • To investigate the real-time metabolic activity of Shigella within host cells.
  • To assess host cell integrity during infection using metabolic markers.

Main Methods:

  • Utilized dynamic Nuclear Polarization-Nuclear Magnetic Resonance (dDNP-NMR) for metabolic analysis.
Keywords:
hyperpolarizationinfectious diseasesintracellular metabolismin‐cell NMRmetabolism

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  • Administered hyperpolarized [2-13C]pyruvate to track its conversion within infected cells.
  • Used hyperpolarized [1,4-13C2]fumarate to [1,4-13C2]malate conversion to assess host cell integrity.

    • Main Results:

    • Successfully demonstrated real-time conversion of [2-13C]pyruvate to [1-13C]acetate within Shigella-infected HeLa cells.
    • Confirmed host cell integrity during infection by observing intact [1,4-13C2]fumarate to [1,4-13C2]malate conversion.
    • Provided direct evidence of Shigella metabolic activity occurring within intact host cells.

    Conclusions:

    • dDNP-NMR is a powerful tool for elucidating metabolic dynamics in bacterial infections.
    • This technique offers deeper insights into host-pathogen metabolic interactions at the cellular level.
    • Findings facilitate the development of targeted therapeutic strategies for infectious diseases.