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Related Concept Videos

Green Algae01:21

Green Algae

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Green algae, also referred to as chlorophytes, are different from red algae in having the chloroplasts containing chlorophylls a and b, which give them their distinct green hue. However, they lack phycobiliproteins, preventing them from developing the red or blue-green pigmentation seen in red algae. In terms of photosynthetic pigment composition, green algae closely resemble plants and share a close evolutionary relationship with them. Taxonomically Green algae belong to Phylum Chlorophyta in...
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Feast-Famine in Cyclic Autotrophy/Heterotrophy Doubles Microalgal Productivity while Controlling Bacterial

Fabrizio Di Caprio1, Flavia Del Signore1, Laura Capobianco1

  • 1Dipartimento di Chimica, Università Sapienza di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy.

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|October 10, 2025
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Summary

This study enhanced microalgal biomass productivity using a "feast-famine" feeding strategy for cyclic autotrophic/heterotrophic cultivation. This method nearly doubled productivity while minimizing bacterial contamination.

Keywords:
Chlorella sorokinianacheese wheycircular economyfeast and faminekinetic modelsluteinprocess controlwastewater treatment

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Area of Science:

  • Biotechnology
  • Microalgal Cultivation
  • Sustainable Energy

Background:

  • Outdoor microalgal cultures face light limitations, reducing daily productivity by ~50% due to nighttime absence of light.
  • Cyclic autotrophic/heterotrophic cultivation offers a potential solution by supplementing organic substrates during dark periods.

Purpose of the Study:

  • To develop and optimize an organic substrate feeding strategy for enhanced microalgal biomass productivity in photobioreactors.
  • To mitigate bacterial contamination issues associated with heterotrophic cultivation.

Main Methods:

  • Implemented a "feast-famine" feeding strategy based on kinetic models for microalgae and bacteria growth.
  • Utilized cyclic autotrophic (day) and heterotrophic (night) cultivation under 12h/12h day/night cycles.
  • Investigated the use of cheese whey as an organic substrate source.

Main Results:

  • The optimized "feast-famine" strategy nearly doubled microalgal biomass productivity from 0.68 to 1.28 g L-1 day-1.
  • Bacterial contamination was kept negligible, comparable to autotrophic controls, with an improved yield (YX/S) of 0.54 g g-1.
  • Direct substrate supplementation at sunset led to significant bacterial contamination and a lower yield (YX/S = 0.18 g g-1).

Conclusions:

  • The "feast-famine" feeding strategy effectively enhances microalgal biomass productivity in cyclic cultivation systems.
  • This approach provides a viable method for controlling bacterial contamination while boosting yield.
  • The study offers guidelines for designing efficient feeding strategies to maximize microalgae production.