Development of a CRISPR/Cas12a-assisted fluorescent aptasensor for simultaneous detection of zearalenone and ochratoxin A
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View abstract on PubMed
Summary
This summary is machine-generated.This study introduces a CRISPR/Cas12a biosensor for simultaneously detecting zearalenone (ZEN) and ochratoxin A (OTA). The novel aptasensor offers sensitive and selective mycotoxin detection in complex samples.
Area Of Science
- Analytical Chemistry
- Biotechnology
- Food Safety
Background
- Mycotoxins like zearalenone (ZEN) and ochratoxin A (OTA) pose significant risks to human and animal health.
- Strict monitoring of mycotoxin contamination in food, feed, and environmental samples is crucial.
- Existing detection methods can be complex and time-consuming.
Purpose Of The Study
- To develop a simple, efficient, and simultaneous detection method for ZEN and OTA.
- To leverage CRISPR/Cas12a technology for enhanced biosensing capabilities.
- To create a fluorescent aptasensor for quantitative mycotoxin analysis.
Main Methods
- A CRISPR/Cas12a-assisted fluorescent aptasensor was designed using a Bifunctional aptamer (B-APT).
- Dual recognition of ZEN and OTA targets was achieved, triggering Cas12a/crRNA cis-cleavage.
- Distinct fluorescent signals were generated for simultaneous and independent quantification.
Main Results
- The aptasensor demonstrated reliable detection of ZEN (0.25 nM - 256 nM) and OTA (1 nM - 1024 nM).
- Limits of detection (LOD) were determined as 190 pM for ZEN and 931 pM for OTA.
- High selectivity and recovery rates (89.17%-109.88% for ZEN, 101.19%-106.51% for OTA) were observed in corn oil samples.
Conclusions
- The developed CRISPR/Cas12a-aptasensor is an effective tool for simultaneous ZEN and OTA detection.
- This methodology offers a sensitive, selective, and efficient approach for mycotoxin monitoring.
- The findings provide valuable insights into simultaneous detection strategies for multiple analytes.
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