Related Experiment Video
Updated: Jan 15, 2026
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
Published on: June 23, 2016
Comparative aptamer screening for patulin and the development of sensitive colorimetric assay for fruit juice
Haijun Pan1, Ruijing Liu2, Dongjie Liu1
1College of Light Industry and Food, Zhongkai University of Agriculture and Engineering/Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou, 510225, China.
Abstract:
A gold nanoparticle-based colorimetric assay was developed for patulin, a mycotoxin, detection in fruit juice. Eight patulin-specific aptamers were rigorously screened using molecular docking and specific binding analyses to select the optimal candidate. The optimized assay exhibited a wide linear detection range of 5.0-200.0 ng/mL in the buffer reaction system and high specificity for patulin. Its reliability for real-world samples was validated by achieving good recoveries (83.6%-90.7%) in spiked apple and hawthorn juice, consistent with HPLC results. This study highlights an effective methodology for screening high-performance aptamers for food contaminants and a novel strategy for high-throughput food safety assessment targeting patulin.
More Related Videos
03:38Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
12:50Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
Published on: June 9, 2014