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Updated: Jun 26, 2026

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
Published on: March 25, 2016
Protocol for the differentiation of HL-60 cells into a neutrophil-like state
Tamara Hornstein1, Klaus Unfried1
1IUF - Leibniz Research Institute for Environmental Medicine, Auf'm Hennekamp 50, 40225 Duesseldorf, Germany.
Abstract:
The human myeloid HL-60 cell line serves as a substitute model in neutrophil research. Here, we present a protocol for HL-60 neutrophil differentiation with combined DMSO/all-trans retinoic acid (ATRA) treatment for 5 days. We describe steps for treating and incubating HL-60 cells. We then detail procedures for harvesting and staining of differentiated HL-60 cells for validation of neutrophil maturation. For complete details on the use and execution of this protocol, please refer to Hornstein et al.1,2.

