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Updated: Jan 15, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
Characterization and discrimination of polysaccharides from three Atractylodes spp. based on multiple structural
Si-Si Jia1, Zi-Wei Han1, Hui-Yang Wang2
1School of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210046, China.
Abstract:
Polysaccharides from 18 batches of Atractylodes lancea (AL), A. chinensis (AC) and A. macrocephala (AM) were compared based on molecular weight (MW) distribution, monosaccharide composition and content, and glycosidic linkage type. Results showed that polysaccharides from AL, AC and AM mainly exhibited three molecular weight fractions, and the average Mw of fraction 1 and 2 in AM is 4.0-1.8 times of AL and AC. Therefore, AM could be distinguished from AL and AC based on large Mw. 7 kinds of monosaccharides were released from AL, AC and AM, and the average content of released monosaccharides in AC (141.2 mg/g) was obviously higher than that in AL (106.4 mg/g) and AM (121.0 mg/g). AC could be distinguished from AL based on monosaccharides content. 1,2-Fruf is the dominant type of glycosidic linkage, which indicated that fructan is abundant. The higher proportion of 1,4-Galp residues after reduction in AM revealed that pectin-type polysaccharides are relatively abundant in AM. Combined with chemometric analysis, the glycosidic linkage can distinguish AL, AC and AM clearly, and 1, 4-Galp and 1,2,4-Rhap residues were confirmed as the differential glycosidic linkages. This study was helpful in understanding the varied functions of different Atractylodes spp. based on chemical composition.
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