Segmented regression modeling of a phytogenic blend on growth and immune function in broilers: a dose-response study

Area of Science:

• Animal Science
• Poultry Nutrition
• Immunology

Background:

• Phytogenic feed additives offer a natural alternative to antibiotics in poultry.
• Understanding optimal dosage is crucial for maximizing benefits and avoiding adverse effects.

Purpose of the Study:

• To investigate the dose-dependent effects of a phytogenic blend (BMX) on broiler chicken performance, immunity, hematology, and organ development.
• To determine the optimal inclusion level of BMX for broiler health and productivity.

Main Methods:

• 300 male broiler chicks were randomly assigned to four treatment groups receiving different concentrations of BMX in drinking water (0.0, 0.25, 0.50, 1.00 mL/L).
• Evaluated growth performance (feed intake, body weight gain, feed efficiency), humoral immunity (antibody titers, immunoglobulin concentrations), hematological parameters, stress indicators (heterophil-to-lymphocyte ratio), and lymphoid organ development.
• Multivariate analysis was used to assess the overall systemic effects of different BMX doses.

Main Results:

• A bell-shaped dose-response curve was observed for growth performance, with the 0.50 mL/L dose showing optimal results.
• Humoral immunity and immunoglobulin levels were significantly enhanced at intermediate BMX doses.
• Hematological profiles, stress indicators, lymphoid organ development, and serum protein concentrations improved at intermediate to high doses, with the 0.50 mL/L dose being most effective.
• The 1.00 mL/L dose exhibited diminishing returns or adverse effects.

Conclusions:

• The phytogenic blend (BMX) demonstrates significant, dose-dependent benefits for broiler chickens.
• The optimal inclusion level for BMX is 0.50 mL/L, enhancing performance, immunity, and systemic health.
• This study provides valuable insights into optimizing phytogenic feed additive strategies in poultry production.