Multi-parameter precise molecular profiling of bladder cancer based on aptamer probe package.

Area of Science:

• Biotechnology
• Molecular Biology
• Cancer Research

Background:

• Precise molecular profiling is crucial for individualized cancer treatment.
• Bladder cancer molecular profiling is essential for diagnosis, treatment, and prognosis.

Purpose of the Study:

• To develop a novel strategy for multi-parameter precise molecular profiling of bladder cancer.
• To create an aptamer probe package for accurate bladder cancer detection and classification.

Main Methods:

• Generation of high-specificity aptamers against bladder cancer using SELEX.
• Construction of a bladder cancer aptamer probe package.
• High-throughput sequencing and flow cytometry for molecular profiling.

Main Results:

• The aptamer probe package effectively distinguished different grades of bladder cancer cells from normal cells.
• High-throughput sequencing results were consistent with flow cytometry.
• The method accurately differentiated bladder cancer patients from healthy individuals and pre-operative from post-operative samples.

Conclusions:

• The aptamer probe package offers a novel, multi-parameter detection method for bladder cancer.
• This strategy enables precise molecular classification, treatment guidance, and prognosis monitoring.
• The approach shows significant potential for clinical application in bladder cancer management.