Bombyx mori nucleopolyhedrosis virus-derived circular RNAs with protein-coding potential facilitate viral replication
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View abstract on PubMed
Summary
This summary is machine-generated.This study reveals that Bombyx mori nucleopolyhedrovirus (BmNPV) produces viral circular RNAs (vcircRNAs) that enhance viral replication. One vcircRNA translates a peptide that suppresses the host
Area Of Science
- Virology
- Molecular Biology
- Genomics
Background
- Circular RNAs (circRNAs) are stable RNA molecules with roles in host-virus interactions.
- Baculoviruses, like Bombyx mori nucleopolyhedrovirus (BmNPV), are insect pathogens.
- Understanding viral circRNAs is crucial for insect immunity research.
Purpose Of The Study
- To investigate the role of circRNAs during BmNPV infection in Bombyx mori.
- To identify and characterize viral circRNAs (vcircRNAs) produced by BmNPV.
- To elucidate the function of vcircRNAs in viral replication and host immune evasion.
Main Methods
- High-throughput sequencing to identify host and viral circRNAs.
- Experimental validation of circRNA existence.
- In vitro translation assays to determine protein production from vcircRNAs.
- Functional assays to assess the impact of vcircRNAs and their encoded peptides on viral replication.
- Mechanism studies focusing on the RNA interference (RNAi) pathway.
Main Results
- Hundreds of host circRNAs and numerous viral circRNAs (vcircRNAs) were identified during BmNPV infection.
- BmNPV encodes vcircRNA-390, which contains an open reading frame (ORF) and internal ribosome entry sites (IRESs).
- vcircRNA-390 is translated into an 81-amino acid viral peptide (VSP81), enhancing viral replication.
- VSP81 targets the host RNA interference (RNAi) pathway, promoting viral immune evasion.
Conclusions
- vcircRNA-390 is a novel proviral factor in BmNPV infections.
- BmNPV utilizes circRNA-mediated translation to enhance viral replication and evade host antiviral defenses.
- The findings expand the understanding of viral circRNA biology and host-pathogen interactions.
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