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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jan 15, 2026

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Published on: February 12, 2019

Flow-LAMP: Label-Free Digital LAMP Using Scatter-Based Flow Cytometry on Vortex-Generated Polydisperse Gel Beads.

Yuchong Zheng^1,2, Wanjun Yao^1,2, Zerui Wu³

¹Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen 518060, China.

Analytical Chemistry

|October 12, 2025

Summary

This summary is machine-generated.

Flow-LAMP is a novel, label-free digital assay for nucleic acid quantification. This cost-effective method uses loop-mediated isothermal amplification and flow cytometry, making digital detection more accessible.

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Area of Science:

Biotechnology
Molecular Diagnostics
Analytical Chemistry

Background:

Accurate nucleic acid quantification is crucial for clinical diagnostics.
Digital PCR adoption is hindered by fluorescence detection and microfluidics.

Purpose of the Study:

To develop a label-free, cost-effective digital nucleic acid detection assay.
To integrate loop-mediated isothermal amplification (LAMP) with flow cytometry for quantification.

Main Methods:

Developed Flow-LAMP assay using vortex emulsification for agarose gel beads.
Utilized flow cytometry (FSC and SSC) to analyze bead volume and amplification.
Magnesium pyrophosphate precipitate indicates positive amplification within gel beads.

Main Results:

Side scatter (SSC) correlated with amplification products; forward scatter (FSC) reflected bead volume.
Achieved a limit of detection of 38.15 copies/μL for Epstein-Barr virus plasmid.
Flow-LAMP results showed strong correlation with qPCR and digital PCR in clinical samples.

Conclusions:

Flow-LAMP provides accurate, accessible digital nucleic acid detection.
Eliminates need for fluorescent labels and specialized microfluidics.
Offers a cost-effective alternative using standard laboratory equipment.