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Spatial Transcriptomic Assessments of Gene Expression Profiles in Human Peri-Implant Lesions
C Dionigi1, A C Ericson1, A Shikhan1
1Department of Periodontology, Institute of Odontology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Journal of Clinical Periodontology
|October 12, 2025
Summary
Peri-implantitis, an inflammatory condition affecting dental implants, shows distinct gene expression patterns. Key biological pathways related to host response and tissue organization are significantly altered in affected sites.
Area of Science:
- Oral biology
- Molecular biology
- Immunology
Background:
- Peri-implantitis is a prevalent complication following dental implant placement.
- Understanding the molecular mechanisms underlying peri-implantitis is crucial for developing effective treatments.
Purpose of the Study:
- To investigate and compare gene expression profiles in human peri-implant tissues with and without peri-implantitis.
- To identify specific biological pathways and gene clusters associated with the condition.
Main Methods:
- Analysis of soft-tissue biopsy samples from patients with severe peri-implantitis and healthy controls.
- Utilized spatial transcriptomics, RNA-sequencing, and immunohistochemistry for comprehensive molecular profiling.
Main Results:
- Identified distinct gene clusters associated with specific tissue compartments in both peri-implantitis and control samples.
- Peri-implantitis tissues exhibited higher overall gene activity and dysregulation of key pathways, including antimicrobial response, collagen organization, and angiogenesis.
- Immunohistochemistry confirmed elevated protein levels for differentially expressed genes identified by transcriptomic analyses.
Conclusions:
- Peri-implant lesions show a clear association between gene expression patterns and tissue compartments.
- The study highlights significant dysregulation of host response pathways in peri-implantitis, indicating an altered molecular landscape in response to bacterial challenges.
