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Updated: May 1, 2026

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Design and Analysis of Untargeted Metabolomics Experiments

Zane G Long1, Rachel W Martin1,2

  • 1Department of Chemistry, University of California, Irvine, California.

Current Protocols
|October 13, 2025
PubMed
Summary

Untargeted metabolomics, using liquid chromatography-tandem mass spectrometry (LC-MS/MS), offers a fast and cost-effective method for identifying unknown small molecules in complex biological samples like plants. This guide provides a comprehensive protocol for researchers new to the technique.

Keywords:
LC‐MS/MSelectrospray ionizationmass spectrometryplant metabolitesuntargeted metabolomics

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