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Updated: Jan 15, 2026

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

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Published on: February 21, 2019

Determining Lysosomal Enzyme Activity Using Fluorogenic Probes.

Etienne Sauvageau¹, Stephane Lefrancois^2,3,4

¹Centre Armand-Frappier Santé Biotechnologie, Institute national de la recherche scientifique, Laval, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|October 13, 2025

Summary

This summary is machine-generated.

This chapter details methods for measuring lysosomal enzyme activity in cells or cell lysates. These assays are crucial for understanding cell biology and developing new therapeutics for lysosomal diseases.

Keywords:

Batten disease Fluorogenic substrates Lysosomal enzyme activity Lysosomes

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Study of Phagolysosome Biogenesis in Live Macrophages

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Published on: March 10, 2014

Area of Science:

Cell Biology
Biochemistry
Molecular Biology

Background:

Lysosomes are vital organelles for cellular degradation.
Lysosomal lumen enzymes are essential for breaking down biological molecules.
Dysfunctional lysosomes are implicated in various diseases.

Purpose of the Study:

To provide detailed methodologies for quantifying lysosomal enzyme activity.
To enable the study of fundamental cellular processes.
To assess the efficacy of therapeutic interventions targeting lysosomal function.

Main Methods:

Measurement of lysosomal enzyme activity in cell lysates.
Assay development for intact cell-based lysosomal enzyme activity detection.

Main Results:

Established protocols for lysosomal enzyme activity assessment.
Demonstrated utility in studying cell biology and therapeutic effects.

Conclusions:

Accurate measurement of lysosomal enzyme activity is feasible in various cellular contexts.
These methods support research in cell biology and drug discovery for lysosomal disorders.