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Updated: Jan 15, 2026

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

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Published on: April 13, 2019

A Fluoride-Specific Riboswitch Biosensor for High-Throughput Enzymatic Defluorination Screening.

Miao Hu^1,2,3, Shefali Bhardwaj⁴, Juan Pinheiro de Oliveira Martinez⁵

¹CSIRO Environment, Black Mountain Science and Innovation Park, Canberra, ACT 2601, Australia.

ACS Synthetic Biology

|October 13, 2025

Summary

This summary is machine-generated.

Researchers developed a novel biosensor for detecting fluoride, crucial for identifying enzymes that break down harmful per- and polyfluoroalkyl substances (PFAS). This tool aids in engineering bioremediation solutions for environmental cleanup.

Keywords:

PFAS bioremediation biosensor enzymatic defluorination fluoride detection riboswitch

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Area of Science:

Environmental Science
Biotechnology
Biochemistry

Background:

Per- and polyfluoroalkyl substances (PFAS) are persistent environmental pollutants due to their chemical stability.
Detecting fluoride release is essential for identifying enzymes capable of defluorination, but current methods lack sensitivity and speed.
There is a need for efficient tools to screen for and engineer bioremediation enzymes targeting PFAS.

Purpose of the Study:

To develop a sensitive and specific biosensor for fluoride detection.
To enable high-throughput screening of enzymes with defluorination capabilities.
To support the engineering of novel bioremediation strategies for PFAS.

Main Methods:

A fluoride-specific riboswitch from *Pseudomonas syringae* was coupled with colorimetric and fluorogenic reporter substrates.
The biosensor was designed for operational simplicity and compatibility with high-throughput screening.
Enzymatic defluorination activity was validated using the defluorinase RPA1163 and various fluorinated substrates.

Main Results:

The developed riboswitch biosensor demonstrated high sensitivity and specificity for fluoride detection.
The biosensor accurately detected enzymatic defluorination across multiple fluorinated compounds, including fluoroacetate and tetrafluoropropanoic acid.
The system proved versatile, cost-effective, and suitable for high-throughput screening applications.

Conclusions:

The fluoride-specific riboswitch biosensor is a powerful tool for identifying and characterizing defluorinase enzymes.
This biosensor facilitates the screening process for enzymes crucial in bioremediation efforts.
The technology supports advancements in engineering enzymes for the biodegradation of persistent fluorinated compounds.