Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Somatosensory, Motor, and Association Cortex

Somatosensory, Motor, and Association Cortex

The somatosensory cortex in the parietal lobes is crucial for interpreting sensory data such as touch, temperature, and proprioception. The somatosensory cortex, situated in the parietal lobes, plays a vital role in interpreting sensory information like touch, temperature, and proprioception—awareness of body position. This specialized brain region features an organized structure wherein neurons at the top primarily process sensations originating from the lower body. In contrast, those at...

Cerebrum: Anatomical Overview II

Cerebrum: Anatomical Overview II

Each cerebral hemisphere can be divided into three main regions. The outermost region, the cerebral cortex, is a thin layer (2 to 4 millimeters thick) made up of gray matter, consisting of neuron cell bodies, dendrites, glial cells, and blood vessels. The middle region, or white matter, is primarily composed of myelinated nerve fibers organized into three types of large tracts: association fibers, commissures, and projection fibers. Association fibers connect different areas within the same...

Cerebellum: Anatomical Regions

Cerebellum: Anatomical Regions

The cerebellum, also known as the "little brain," is located in the posterior cranial fossa, inferior to the tentorium cerebelli and dorsal to the brainstem. It plays a significant role in motor control, coordination, and proprioception.
Cerebellar Structure
Externally, the cerebellum features a highly convoluted surface with numerous folia (narrow ridges) separated by shallow sulci (grooves). The cerebellum is divided into two hemispheres by a thin median structure known as the vermis. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Glutamatergic neuron-tumor synapses shape human glioblastoma cell states through radial glia plasticity.

bioRxiv : the preprint server for biology·2026

Same author

Integrated clinical and postmortem profiling in schizophrenia reveals a cognitive subtype linked to cerebrovascular disease.

Translational psychiatry·2026

Same author

The subiculum: cell-type-specific composition, computation, and function.

Trends in neurosciences·2026

Same author

Divergent hippocampal output via covariant local and long-range neuronal structure.

Progress in neurobiology·2026

Same author

Omics-based approaches for acute brain injury research.

Current opinion in critical care·2026

Same author

Quantification and analysis of multiplexed fluorescence insitu hybridization data using open-source tools.

STAR protocols·2025

Same journal

Deletion of Fbxo25 causes excessive repetitive behavior, impaired recognition memory, reduced dendritic complexity, and aberrant protein expression in mice.

Progress in neurobiology·2026

Same journal

Neuropsychiatric Disease Mechanisms and Interventions. from 22q11.2 Deletion Syndrome Experimental Studies.

Progress in neurobiology·2026

Same journal

REM sleep as a dummy-model of the world: A theoretical framework.

Progress in neurobiology·2026

Same journal

CA3 transiently modulates spatial representation in CA1.

Progress in neurobiology·2026

Same journal

Love, death, and oxytocin: In memory of Larry Young.

Progress in neurobiology·2026

Same journal

Assessing peripheral oxytocin and cortisol levels and epigenetic variations of oxytocin receptor and glucocorticoid receptor genes in school-aged preterm-born children.

Progress in neurobiology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 15, 2026

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

Distinct Layer 6b transcriptomic subtypes parcellate the cortical mantle.

Margarita Kapustina¹, Brianna N Bristow¹, Mark S Cembrowski²

¹Dept. of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Boulevard, Vancouver, BC, Canada.

Progress in Neurobiology

|October 13, 2025

Summary

This summary is machine-generated.

Researchers mapped Layer 6b (L6b) neuron subtypes in the neocortex using advanced transcriptomics. They discovered distinct L6b subtypes with unique spatial patterns, challenging traditional views of their location and identity.

Keywords:

Cell types Layer 6b, single-cell RNA sequencing Multiplexed fluorescent in situ hybridization Single-cell spatial transcriptomics

More Related Videos

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Related Experiment Videos

Last Updated: Jan 15, 2026

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Area of Science:

Neuroscience
Genomics
Cell Biology

Background:

Layer 6b (L6b) neurons are crucial for brain function but their subtypes and spatial distribution remain unclear.
Previous characterizations described L6b neurons as a thin layer in the deep neocortex.

Purpose of the Study:

To comprehensively characterize L6b neuron identity, molecular heterogeneity, and spatial organization.
To identify distinct L6b subtypes and map their distribution across the cortical mantle.

Main Methods:

Single-cell RNA sequencing
Highly multiplexed fluorescent in situ hybridization
Single-cell spatial transcriptomics on 450,496 cells

Main Results:

Identified multiple distinct L6b subtypes with unique molecular signatures.
Revealed a patchwork-like spatial organization of L6b subtypes across the cortex, with some extending beyond the deep cortical layers.
Showed L6b neurons can be transcriptionally distinct yet spatially intermingled with Layer 6a neurons.

Conclusions:

Provides the most comprehensive cellular phenotyping of L6b neurons to date.
Establishes a spatial-molecular framework for understanding L6b neuron function in brain health and disorder.
Guides future research on L6b cell subtypes and their roles in neurological conditions.