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The effect of storage temperature and time before centrifugation on coagulation parameters in neonatal Japanese Black

Riko Ito1,2, Keinosuke Sanada2, Satoshi Inoue1

  • 1Graduate School of Veterinary Sciences, Iwate University, Iwate, Japan.

The Journal of Veterinary Medical Science
|October 13, 2025
PubMed

This study aimed to determine the effects of precentrifugation storage time and temperature on coagulation and fibrinolysis parameters in Japanese Black calves. Blood samples were collected from calves under 6 weeks of age into plastic tubes containing 3.2% sodium citrate. Whole blood was stored at either room temperature or under refrigeration prior to centrifugation. Prothrombin time, activated partial thromboplastin time, fibrinogen level, and antithrombin level were measured. The results indicated that plasma parameters in samples centrifuged within 9 hr of collection showed no more than ± 10% variation compared with those in samples centrifuged within 1 hr of collection.

Keywords:
activated partial thromboplastin timeantithrombincalffibrinogenprothrombin time

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