Related Experiment Video

Updated: Jan 15, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.7K

TREX-2 mRNA Export Complex Interacts with HLB Component FLASH and Is Recruited to Processed Histone mRNAs

M M Kurshakova1, Y A Yakusheva2, S G Georgieva2

  • 1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. kursha@mail.ru.

Doklady. Biochemistry and Biophysics
|October 13, 2025
PubMed

Insights

The TREX-2 complex interacts with FLASH, a key protein in histone mRNA processing. This interaction facilitates the nuclear export of histone mRNAs, crucial for regulating gene expression and preventing disease.

Area of Science:

  • Molecular Biology
  • Gene Expression Regulation
  • Cellular Biology

Background:

  • Histone gene expression is vital for cellular function and its disruption causes pathologies.
  • Nuclear mRNA export is a critical step in gene expression, primarily mediated by the TREX-2 complex for poly(A)-containing mRNAs.
  • Histone mRNAs, unlike most mRNAs, lack poly(A)-tails but are known to be exported from the nucleus.

Purpose of the Study:

  • To investigate the interaction between the TREX-2 protein complex and the machinery involved in histone mRNA processing.
  • To elucidate the mechanism by which TREX-2 participates in the nuclear export of histone mRNAs.

Main Methods:

  • Co-immunoprecipitation assays to detect protein-protein interactions.
  • Analysis of TREX-2 association with histone mRNA processing factors.
  • Studying the role of FLASH in TREX-2 recruitment to histone mRNAs.

Main Results:

  • TREX-2 directly interacts with the FLASH protein.
  • FLASH is identified as a key component of the specialized histone mRNA processing machinery and the histone locus body (HLB).
  • The interaction between TREX-2 and FLASH mediates the recruitment of the TREX-2 complex to processed histone mRNAs.

Conclusions:

  • TREX-2 collaborates with FLASH to ensure the efficient nuclear export of histone mRNAs.
  • This interaction highlights a specialized pathway for the export of non-polyadenylated histone mRNAs.
  • Understanding this mechanism provides insights into the regulation of histone gene expression and its implications in disease.
Keywords:
ENY2FLASHHLBORCPCID2TREX-2Xmas-2histone genesmRNA export

More Related Videos

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

7.7K
Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection11:32

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Published on: December 4, 2010

15.8K

Related Concept Videos

Nuclear Export01:42

Nuclear Export

The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
4.8K
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.9K
Regulated mRNA Transport02:22

Regulated mRNA Transport

3.3K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.1K
Transcription Elongation Factors02:35

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
13.4K
Transcription Elongation Factors02:35

Transcription Elongation Factors

4.5K