Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Gene Regulation During Sporulation

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Regulation of spikelet number during wheat spike development.

Current opinion in plant biology·2026

Same author

UCHL3 Promotes Triple-Negative Breast Cancer Metastatic Potential Through Enhancing Cell Migration and Invasion.

Applied biochemistry and biotechnology·2026

Same author

Regulation of spikelet number during wheat spike development.

bioRxiv : the preprint server for biology·2026

Same author

Host immune dysfunction and Mycoplasma pneumoniae immune evasion: dual drivers of pathogenesis in Mycoplasma pneumoniae pneumonia.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2026

Same author

Neutrophil-to-lymphocyte ratio as an independent predictor of severe pertussis in children: A 17-year retrospective cohort study.

Journal of infection and public health·2026

Same author

MicroRNA156 and its targeted SPL genes interact with the photoperiod, vernalization, and gibberellin pathways to regulate wheat heading time.

The Plant journal : for cell and molecular biology·2026

Search research articles

Related Experiment Video

Updated: Jan 15, 2026

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

Spatial and single-cell expression analyses reveal complex expression domains in early wheat spike development.

Xiaosa Xu¹, Huiqiong Lin^2,3, Junli Zhang²

¹Department of Plant Biology, University of California, Davis, CA, 95616, USA. xjkxu@ucdavis.edu.

|October 14, 2025

Summary

This study maps wheat spike development using single-molecule fluorescence in situ hybridization (smFISH) and single-cell RNA sequencing (scRNA-seq). It reveals gene expression patterns and identifies key genes regulating wheat spike and spikelet development for improved crop productivity.

Keywords:

Co-expression analysis Gene expression Single-cell RNA sequencing Single-molecule fluorescence in situ hybridization Spatial transcriptomics Spike development Transcription factors Wheat

More Related Videos

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

Related Experiment Videos

Last Updated: Jan 15, 2026

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

Area of Science:

Plant Biology
Molecular Biology
Genomics

Background:

Wheat is crucial for global food security.
Understanding molecular mechanisms of wheat spike and spikelet development is key to improving crop yields.

Purpose of the Study:

To create a comprehensive atlas of cell clusters and gene expression domains during early wheat spike development.
To identify and characterize genes involved in regulating wheat spike and spikelet development.

Main Methods:

Integration of single-molecule fluorescence in situ hybridization (smFISH) and single-cell RNA sequencing (scRNA-seq).
Spatiotemporal expression analysis of 99 genes across 48,225 cells at key developmental stages (W1.5, W2.5, W3.5).
scRNA-seq profiling of 26,009 cells, imputation of gene expression, and co-expression analyses.

Main Results:

Identification of 21 distinct expression domains and 23 cell clusters during wheat spike development.
Characterization of spatiotemporal gene expression patterns, including LFY, SPL14, and FZP, with revealed functional roles.
Generation of a public website for visualizing imputed gene expression in spatially anchored cells.

Conclusions:

smFISH and scRNA-seq offer powerful complementary approaches for dissecting gene networks in wheat spike development.
The study provides a valuable resource for identifying novel co-expressed genes for future functional studies and crop improvement.