Pluronic Acid Prevents Sorter-Induced Cell Stress in Myeloid Cells From Mouse Peritoneal Cavity

Sorter-induced cell stress (SICS) is a recognized challenge within the fluorescence-activated cell sorting (FACS) community; however, it remains significantly underreported in the scientific literature, with limited studies proposing effective mitigation strategies. In the present study, we observed that myeloid cells isolated from the peritoneal cavity exhibited heightened sensitivity to FACS-induced stress relative to other immune cell populations. Notably, we identified that the addition of pluronic acid to cell suspensions prior to sorting substantially improved post-sort cell viability. These findings suggest that pluronic acid may represent a broadly applicable approach to preserving the viability of sensitive cell types, potentially extending to cells derived from other tissues.