The Na+/K+-ATPase β1 Subunit is a Kidney ADP-Ribosyl Cyclase
Tae-Sik Nam1, Seong-Kyu Choe2,3, Uh-Hyun Kim1
1Department of Biochemistry, Wonkwang University, Iksan, Korea.
Abstract:
ADP-ribosyl cyclase (ARC) produces a Ca2+-mobilizing second messenger, cyclic ADP-ribose (cADPR), from NAD. In this study, we purified an ARC from rat kidney, which was identified as the β1 subunit of Na, K-ATPase (Atp1b1). Recombinant Atp1b1 exhibited ARC activity and generated cADPR. Knockdown of Atp1b1 in mouse mesangial cells (MES-13) inhibited angiotensin II (AngII)-induced cADPR synthesis and the subsequent long-lasting Ca2+ signals. These findings demonstrate that Atp1b1 is coupled with the AngII receptor and is activated to produce cADPR, thereby mediating Ca2+ signaling in the kidney.
More Related Videos
15:43Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
Published on: January 7, 2013
08:46Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
Published on: September 1, 2015
Related Concept Videos
ATP Driven Pumps III: V-type Pumps
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
ATP Synthase: Structure
ATP Driven Pumps II: P-type Pumps
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
ATP Synthase: Mechanism
The ADP/ATP Carrier Protein
Allosteric Proteins-ATCase
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
