Chromosome-level genome assembly of the sea cucumber, Colochirus anceps

Colochirus anceps is a benthic sea cucumber recognized for its striking aposematic coloration, ecological role in nutrient recycling, and biomedical potential. In this study, we utilized Illumina short-read, PacBio HiFi long-read, and Hi-C sequencing technologies to assemble the chromosome-level genome of C. anceps. This represents the first genome assembly for the order Dendrochirotida within the class Holothuroidea. The assembled genome has a total size of 2,238.33 Mb, with a contig N50 of 15.09 Mb, and 95.09% of the assembly sequences have been anchored to 23 chromosomes. Annotation identified 24,102 protein-coding genes and revealed a high repeat content of 70.95%. The genome achieved a completeness score of 94.3%, as evaluated through BUSCO analysis. This genome assembly provides valuable insights into the genomic architecture and evolutionary dynamics of sea cucumbers, laying a foundation for exploring their unique biological traits, adaptability, and diversity.