Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Receptor-mediated Endocytosis01:39

Receptor-mediated Endocytosis

110.5K
Overview
110.5K
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

7.5K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
7.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design of Hyperporous Molecularly Imprinted Thin Films for Ultrasensitive Antibody-Free QCM Detection of a Small-Cell Lung Cancer Biomarker.

ACS sensors·2026
Same author

Development of a gene-activated scaffold using niosomes and minicircle DNA as a novel non-viral delivery platform to enhance cartilage repair.

Colloids and surfaces. B, Biointerfaces·2026
Same author

Photo-switchable supramolecular glycochips for capturing suspension tumor cells and real-time profiling of cell surface glycosylation.

The Analyst·2026
Same author

CX3CR1<sup>+</sup> synovial macrophages accumulate in the joint during experimental hemophilic arthropathy but are not required for acute synovitis.

Journal of thrombosis and haemostasis : JTH·2026
Same author

Navigating the <i>Nanoverse</i>: how emerging nanomaterials are transforming bioscience and society.

Nanoscale advances·2026
Same author

Monovalent antibody treatment of von Willebrand disease.

Blood advances·2026

Related Experiment Video

Updated: Jan 15, 2026

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization
10:06

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Published on: September 2, 2022

2.2K

Terminal sialic acids in the nanoparticle corona modulate cellular uptake.

Marko Dobricic1, Alberto Martinez-Serra1, Claudia Durall2

  • 1Department of Chemistry, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland.

Communications Chemistry
|October 14, 2025
PubMed
Summary

Nanoparticle (NP) interactions with the body are influenced by their biomolecular corona. The glycosylation of this corona, particularly sialic acid content, affects NP stability and cellular interactions, impacting their biological fate.

More Related Videos

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface
13:36

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

Published on: October 20, 2023

4.2K
Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

7.9K

Related Experiment Videos

Last Updated: Jan 15, 2026

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization
10:06

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Published on: September 2, 2022

2.2K
Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface
13:36

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface

Published on: October 20, 2023

4.2K
Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

7.9K

Area of Science:

  • Nanomedicine
  • Biomaterials Science
  • Glycobiology

Background:

  • Nanoparticles (NPs) engineered for therapy interact with biological systems, forming a biomolecular corona of adsorbed biomolecules.
  • The glycosylation (sugar coating) of this corona is increasingly recognized as a critical factor influencing NP behavior in vivo.
  • The specific role of corona glycosylation, especially its interaction with cellular receptors, remains incompletely understood.

Purpose of the Study:

  • To investigate how the glycan composition of the nanoparticle biomolecular corona affects NP-receptor interactions.
  • To determine the impact of sialic acid content within the corona's glycans on NP stability and cellular engagement.
  • To establish advanced parameters for predicting nanoparticle biological fate based on corona glycosylation.

Main Methods:

  • Enzymatic approaches were used to dissect the glycan composition of the biomolecular corona.
  • Differential centrifugal sedimentation and quartz crystal microbalance were employed to analyze NP-corona interactions with isolated glycan receptors.
  • Flow cytometry was utilized to confirm these interactions in relevant cell lines.

Main Results:

  • Differences in monosaccharide sialic acid content within the corona significantly altered NP-corona interactions with specific glycan receptors.
  • These changes in NP-corona-receptor interactions were validated in relevant cell lines using flow cytometry.
  • The study demonstrates a direct link between corona glycosylation and NP interaction dynamics.

Conclusions:

  • The glycosylation of the biomolecular corona plays a crucial role in nanoparticle-biological interactions.
  • Sialic acid content is a key determinant of how nanoparticle coronas interact with cellular receptors.
  • Understanding corona glycosylation provides advanced predictive parameters for nanoparticle behavior and therapeutic efficacy.