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Related Experiment Videos

The SC5b-7 complex: formation, isolation, properties, and subunit composition.

E R Podack, W P Kolb, H J Müller-Eberhard

    Journal of Immunology (Baltimore, Md. : 1950)
    |December 1, 1977
    PubMed
    Summary
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    The complement system

    Area of Science:

    • Biochemistry
    • Immunology

    Background:

    • The complement system is a crucial part of innate immunity.
    • Activation of complement component C5 leads to the formation of the membrane attack complex (MAC).

    Purpose of the Study:

    • To characterize a soluble complex formed during complement activation.
    • To identify the components and properties of this complex.

    Main Methods:

    • Isolation and characterization of a soluble C5-C7 complex.
    • Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to analyze protein composition.
    • Analysis of neoantigen expression.

    Main Results:

    • A soluble complex of C5, C6, and C7 was formed in C8-depleted serum.

    Related Experiment Videos

  • This complex contained an 88,000-dalton glycoprotein, identified as S-protein (also known as complement component 8 binding protein).
  • S-protein is incorporated during the formation of the SC5b-7 complex, not binding to C5b-6 alone.
  • The SC5b-7 complex shares a neoantigen with SC5b-9 but possesses a distinct neoantigen compared to C5b-6.

    • Conclusions:

    • S-protein is a component of the soluble C5b-7 complex.
    • The incorporation of S-protein influences the antigenic properties of the complex.
    • This finding provides insight into the assembly and function of complement complexes.