Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...

Combination Therapies and Personalized Medicine

Combination Therapies and Personalized Medicine

Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Self-Monitoring Single-Atom Copper Nanocapsule for Cascade-Responsive Tumor Cuproptosis Therapy.

Angewandte Chemie (International ed. in English)·2026

Same author

Optimizing the mucosal frontier: longevity and epithelial barriers in prime-boost mRNA vaccine strategies.

Journal of translational medicine·2026

Same author

Network analysis of anxiety and depression in college students with different levels of physical activity.

Scientific reports·2026

Same author

Development of a Machine Learning‑Based Prognostic Model for Intermediate Trophoblastic Tumors: A Single-Center Study With Web-Based Tool Implementation.

JCO precision oncology·2026

Same author

Artificial MetalloDNAzymes with High-Density, Near-Atomic Precision Organization of Metal Cofactors for Enhanced Bioorthogonal Catalysis.

Journal of the American Chemical Society·2026

Same author

Amplification of Endoplasmic Reticulum Stress via Inhibiting Lipid Droplet Formation to Enhance Chemodynamic Immunotherapy.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

Carbonylative Aminative Suzuki-Miyaura Coupling: Pd-Catalyzed Synthesis of Amides from Vinyl/Aryl Halides and Boronic Acids.

Journal of the American Chemical Society·2026

Same journal

Divergent Asymmetric Synthesis of Glutinosasins A-E.

Journal of the American Chemical Society·2026

Same journal

Ultrastrong Polyketone Hot-Melt Adhesives Enabled by Ni-Catalyzed Carbonylative Polymerization.

Journal of the American Chemical Society·2026

Same journal

Programmable Anomalous Photovoltaics Enabled by Light-Electric Dual-Field Control.

Journal of the American Chemical Society·2026

Same journal

Biomimetic Redox-Mediated Proton Relay in Nanoreactors for Photocatalysis.

Journal of the American Chemical Society·2026

Same journal

The Sulfur Monoxide-Water Complex.

Journal of the American Chemical Society·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 15, 2026

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Published on: February 3, 2015

In Situ PROTAC Synthesis Enabled by Pathologically Activated Bioorthogonal Catalysis for Precision Cancer Therapy.

Dilan Ouyang¹, Rongjin Yang¹, Yuhang Yao¹

¹New Cornerstone Science Laboratory, MOE Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou 350108, P. R. China.

Journal of the American Chemical Society

|October 15, 2025

Summary

This summary is machine-generated.

This study introduces a new method for synthesizing Proteolysis-targeting chimeras (PROTACs) inside cancer cells. This approach enables targeted protein degradation, enhancing cancer treatment efficacy and reducing side effects.

More Related Videos

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

Related Experiment Videos

Last Updated: Jan 15, 2026

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Published on: February 3, 2015

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

Area of Science:

Biochemistry
Molecular Biology
Drug Discovery

Background:

Proteolysis-targeting chimeras (PROTACs) offer therapeutic potential by degrading target proteins via the ubiquitin-proteasome system.
Current PROTAC applications are limited by poor cell permeability and off-target effects, necessitating novel delivery and synthesis strategies.

Purpose of the Study:

To develop a modular strategy for in situ PROTAC synthesis within cancer cells using bioorthogonal catalysis.
To enhance targeted protein degradation and minimize systemic toxicity associated with conventional PROTACs.

Main Methods:

Developed a platform integrating glutathione-activated Click-T-Cu(II) complexes and fragmented PROTAC precursors.
Utilized AS1411 aptamer-conjugated liposomes for targeted delivery to nucleolin-overexpressing cancer cells.
Employed click chemistry for intracellular PROTAC assembly and subsequent oncoprotein degradation.

Main Results:

Demonstrated efficient in situ PROTAC synthesis and targeted protein degradation in vitro and in vivo.
Achieved robust antitumor activity with high selectivity and favorable biocompatibility.
Showcased modularity by degrading BRD4, PARP1, and NF-κB using diverse warheads.

Conclusions:

The pathologically activated bioorthogonal catalysis PROTAC (ABC-PROTAC) strategy enables precise, targeted protein degradation.
This approach significantly reduces systemic toxicity compared to conventional PROTACs.
The modular ABC-PROTAC platform holds promise for advanced cancer therapeutics.