Enhanced microbial production of pyridoxine (Vitamin B6) in Bacillus subtilis via pathway and process optimization
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View abstract on PubMed
Summary
This summary is machine-generated.This study engineered Bacillus subtilis to produce more pyridoxine (PN), a form of vitamin B6, through genetic modifications and optimized fermentation. The engineered strain achieved a record high PN yield of 174.6 mg/L.
Area Of Science
- Biotechnology
- Microbial Engineering
- Metabolic Engineering
Background
- Vitamin B6, specifically pyridoxine (PN), is vital for numerous physiological processes.
- Microbial fermentation offers a sustainable route for PN biosynthesis, but low yields from natural strains hinder industrial application.
- Enhancing PN production in microbial systems is crucial for meeting demand.
Purpose Of The Study
- To develop a high-yielding strain of pyridoxine (PN) using Bacillus subtilis.
- To improve PN production through a combination of pathway engineering and fermentation process optimization.
- To establish a new record for PN biosynthesis in B. subtilis.
Main Methods
- Overexpression of key genes (epd, pdxB, serC, pdxA, pdxJ) in the deoxyxylulose-5-phosphate (DXP)-dependent pathway.
- Screening and optimization of ribosome binding site (RBS) sequences for DXP-independent pathway genes (pdxST).
- Systematic medium optimization and fed-batch fermentation for enhanced PN biosynthesis.
Main Results
- Overexpression of DXP-dependent pathway genes increased PN titer by 3.2-fold to 2.9 mg/L.
- Optimization of DXP-independent pathway genes and RBS sequences further boosted PN titer to 24.6 mg/L.
- Medium optimization and fed-batch fermentation resulted in a record PN titer of 174.6 mg/L in B. subtilis.
Conclusions
- Pathway engineering and medium optimization are effective strategies for significantly improving PN production.
- The developed high-yielding B. subtilis strain offers a promising platform for industrial PN biosynthesis.
- This study provides valuable insights for the large-scale production of vitamin B6 through microbial fermentation.
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