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Published on: July 27, 2022
Carpesium abrotanoides ethanol extract alleviates dextran sulfate sodium-induced ulcerative colitis by suppressing
Aejin Kim1,2, So Yeon Kim3, Kyuhyung Jo1
1KM Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea.
Background:
Carpesium abrotanoides has been traditionally used to treat various inflammatory and infectious diseases. However, there is no scientific report on its protective activity against intestinal inflammatory disorders, including ulcerative colitis (UC). In this study, we aimed to investigate the mechanisms underlying the protective effects of C. abrotanoides extract (CAE) in UC treatment.
Materials And Methods:
Key components of CAE were identified through ultra-performance liquidchromatography, and their potential targets and pathways were predicted through network pharmacology and molecular docking. The therapeutic effects of CAE were evaluated in a dextran sulfate sodium-induced UC mouse model by assessing clinical parameters, colon length, histopathology, and the expression of inflammatory, tight junction, and apoptosis-related markers.
Results:
The components of CAE, including chlorogenic acid, kaempferol 3-O-rhamnoside, 1,3-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid, were identified. These components interacted with critical targets, including tumor necrosis factor, interleukin-6, interleukin-1β, caspase-3, and Bcl-2, modulating inflammatory and apoptotic pathways. In vivo experiments showed that CAE reduced the disease activity index, prevented colon shortening, and ameliorated histological damage. It preserved tight junction proteins (ZO-1 and claudin-1), reduced inflammatory cell infiltration, and downregulated pro-inflammatory mediators. Moreover, CAE inhibited the expression of the pro-apoptotic protein Bax, the execution-phase apoptotic markers cleaved caspase-3 and cleaved PARP, while upregulated the expression of the anti-apoptotic protein Bcl-2.
Conclusion:
CAE alleviates dextran sulfate sodium-induced colitis by exerting anti-inflammatory and anti-apoptotic effects and maintaining intestinal barrier integrity. These findings support the potential of CAE as a natural multitarget therapeutic agent for UC.
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