[Error Correction Techniques in Synthetic Oligonucleotides and Synthetic DNA]
A N Sinyakov1,2, E V Kostina1
1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
Abstract:
Methods for correcting errors in synthetic oligonucleotides and genetic constructs derived from them are described. Methods for isolating oligonucleotides with a perfect structure from oligonucleotide pools are discussed. Methods for correcting errors in the DNA structure using mismatch-specific endonucleases and proteins of the bacterial DNA repair system are considered. Examples of practical application of the methods developed for adjusting the structure of synthesized genetic constructs are given.
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