MiR-22-3p regulates the SIRT1/P53 signaling pathway, thereby influencing ferroptosis in diffuse large B-cell lymphoma...

Diffuse large B-cell lymphoma (DLBCL) is an aggressive non-Hodgkin lymphoma with high mortality. Ferroptosis, an iron-dependent form of programmed cell death, has potential in cancer therapy. This study investigates the role of miR-22-3p in regulating ferroptosis in DLBCL through the SIRT1/P53 pathway. Bioinformatics analysis identified key microRNAs and target genes associated with ferroptosis. Dual-luciferase assays confirmed the interaction between miR-22-3p and its target genes, while qPCR and Western blot demonstrated its regulatory effects on the SIRT1/P53 axis. Immuno-precipitation revealed the interaction between SIRT1 and P53. MiR-22-3p expression was found to be downregulated in DLBCL patients. MiR-22-3p mimic promoted apoptosis and inhibited proliferation and invasion of DLBCL cells. Knockdown of SIRT1 disrupted mitochondrial morphology, and miR-22-3p was shown to trigger ferroptosis via the SIRT1/P53 pathway.