Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The amino acid transporter LAT1 coordinates proper motor function at the perinatal stage.

Cell death & disease·2026
Same author

MMP10 is highly expressed in an osteosarcoma stem cell model and predicts poor prognosis.

Gene·2026
Same author

Regulatory role of mTORC1 signaling in osteoblasts in acute myeloid leukemia progression and steady-state hematopoiesis.

iScience·2026
Same author

Protein Posttranslational Modifications in Glioma Stem Cells.

Biological & pharmaceutical bulletin·2025
Same author

Evaluation of an <sup>211</sup>At-labeled RGD peptide in an orthotopic glioblastoma model by targeted alpha therapy.

Nuclear medicine and biology·2025
Same author

Liposomes Carrying Surface-Conjugated Trypsin for Controlled Proteolysis Reactions.

ACS omega·2025

Related Experiment Video

Updated: Jan 15, 2026

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
09:17

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

2.7K

Integrated Spatial and Single-Cell Transcriptomics Reveals Poor Prognostic Ligand-Receptor Pairs in Glioblastoma.

Makoto Yoshimoto1, Kengo Sugihara1, Kazuya Tokumura1

  • 1Department of Bioactive Molecules, Pharmacology, Gifu Pharmaceutical University, Gifu 501-1196, Japan.

Cells
|October 15, 2025
PubMed
Summary

Cell-cell interactions in glioblastoma (GBM) involve ligand-receptor pairs that impact prognosis. This study identified 13 poor-prognosis LR pairs, mainly in extracellular matrix processes, localized within tumor tissues, suggesting potential therapeutic targets.

Keywords:
Chinese glioma genome atlascell–cell interactiongene expression omnibusglioblastomaspatial transcriptomicsthe cancer gnome atlas

More Related Videos

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

6.0K
Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
05:45

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Published on: July 31, 2017

10.1K

Related Experiment Videos

Last Updated: Jan 15, 2026

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
09:17

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

2.7K
Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

6.0K
Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
05:45

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Published on: July 31, 2017

10.1K

Area of Science:

  • Oncology
  • Molecular Biology
  • Bioinformatics

Background:

  • Glioblastoma (GBM) is a lethal brain tumor where cell-cell interactions (CCIs) within the tumor microenvironment influence patient outcomes.
  • Ligand-receptor (LR) pairs mediate CCIs, but their specific prognostic value and spatial distribution in GBM are not fully understood.

Purpose of the Study:

  • To identify prognostic ligand-receptor (LR) pairs in glioblastoma (GBM).
  • To investigate the intratumoral localization of these LR pairs using multitranscriptomic analysis.

Main Methods:

  • Comprehensive evaluation of CCIs using single-cell RNA sequencing data from 40,958 cells.
  • Identification of intercellular networks formed by specific LR pairs.
  • Survival analysis to determine prognostic significance of LR pairs.
  • Spatial transcriptomic analysis across three independent GBM cohorts.

Main Results:

  • CCIs in GBM form intercellular networks mediated by specific LR pairs, primarily involved in extracellular matrix (ECM) biological processes.
  • Thirteen LR pairs associated with ECM processes were identified as contributing to poor prognosis in GBM patients (p < 0.05).
  • Spatial transcriptomic analysis confirmed the specific localization of these poor-prognostic LR pairs within GBM tissues.

Conclusions:

  • Specific LR pairs, particularly those in ECM-related pathways, are linked to poor prognosis in glioblastoma.
  • The spatial distribution of these prognostic LR pairs within tumor tissues provides insights into GBM heterogeneity.
  • These findings highlight potential novel therapeutic targets for glioblastoma treatment.