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Updated: Jan 15, 2026

The Soft Agar Colony Formation Assay
Published on: October 27, 2014
RNF121 promotes the proliferation, migration, and invasion of lung cancer cell lines
Jun Jiang1, Xiaoshuai Chen1, Xinjie Dong1
1Department of Pathology, Lequn Branch, The First Hospital of Jilin University, Changchun, China.
Objectives:
RING finger proteins are a large family of proteins within the human genome that play essential roles in the regulation of physiological processes and have been implicated in cancer progression. However, the relationships among RING Finger Protein 121 (RNF121), E3 ubiquitin ligase, and tumorigenesis have yet to be determined. In this study, we aimed to comprehensively elucidate the functions of RNF121 in non-small cell lung cancer (NSCLC).
Methods:
On the basis of MTT, colony formation, and Transwell assays, we evaluated RNF121-specific functions that were found to be closely associated with clinicopathological features.
Results:
RNF121 expression was demonstrated to be closely associated with different diseases. By altering the levels of this protein in the A549 and H1299 cell lines, we discovered that this protein promotes cell proliferation, migration, and invasion.
Conclusion:
This study is the first to demonstrate that RNF121 plays a prominent role in driving NSCLC progression and that its expression is correlated with clinicopathological features. Accordingly, this gene would serve not only as a key prognostic indicator but also as a novel potential therapeutic target for the treatment of NSCLC.
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