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Related Experiment Video

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Supramaximal Intensity Hypoxic Exercise and Vascular Function Assessment in Mice
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Establishing VO2peak-based exercise intensity classification and physiological validation in ICR mice.

Deunsol Hwang1, Sunghwan Kyun1, Inkwon Jang1,2

  • 1Physical Activity and Performance Institute, Konkuk University, Seoul, Republic of Korea.

Physical Activity and Nutrition
|October 15, 2025
PubMed
Summary

This study established a standardized VO2peak framework to classify exercise intensity in ICR mice. Fixed-incline protocols reduce variability, providing reliable speed thresholds for research and interventions.

Keywords:
ICR miceVO2peakVO2reserveblood lactateconstant-load exercise testenergy metabolismgraded exercise test

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Area of Science:

  • Exercise Physiology
  • Animal Models
  • Metabolic Research

Background:

  • Accurate exercise intensity classification is crucial for rodent research.
  • Standardized protocols are needed to ensure reproducibility in mouse studies.
  • VO2peak (maximal oxygen consumption) is a key metric for exercise physiology.

Purpose of the Study:

  • To develop a VO2peak-based framework for classifying exercise intensity in ICR mice.
  • To validate physiological responses associated with different exercise intensities.
  • To establish reliable speed thresholds for exercise interventions in mice.

Main Methods:

  • Four maximal graded exercise test (GXT) protocols were performed on male ICR mice.
  • VO2peak was measured using a treadmill-equipped metabolic chamber.
  • Constant-load tests (CXT) were used to assess metabolic responses and blood lactate at varying intensities (40%, 60%, 80% VO2reserve).

Main Results:

  • VO2peak values were comparable across most GXT protocols, with fixed-incline protocols showing less variability.
  • Oxygen consumption and energy expenditure increased with exercise intensity during CXT.
  • Blood lactate levels rose with intensity, exceeding the aerobic-anaerobic threshold at 60% VO2reserve.

Conclusions:

  • Standardized VO2peak reference values were established for classifying exercise intensity in ICR mice.
  • Fixed-incline GXT protocols are recommended for reduced variability in mouse exercise testing.
  • The derived speed thresholds provide a foundation for future exercise interventions and research in mice.